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Effect of Deltamethrin and a Neem Based Pesticide Achook on Some Biochemical Parameters in Tissues Liver, Ovary and Muscle of Zebrafish Danio rerio (Cyprinidae)

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Author Affiliations

  • 1Zebrafish Laboratory Department of Zoology, DDU Gorakhpur University, Gorakhpur (UP) INDIA

Res.J.chem.sci., Volume 1, Issue (4), Pages 125-134, July,18 (2011)

Abstract

The present study is aimed to evaluate the changes in total protein, total free amino acid content and nucleic acids (DNA and RNA) in the liver, ovary and muscle of Zebrafish after exposure to 96 h LC, LC10 and LC20 of Deltamethrin and neem based pesticide Achook. It was found that the protein content was reduced to 45, 68, 65% (after Deltamethrin exposure) and 54, 81, 85% (after Achook exposure) as compared to the controls (100%) after 16 days in the liver, ovary and muscle, respectively at LC20 exposure. The total free amino acid contents in liver were enhanced to 146 and 142% of controls (100%) after exposure to LC20 of Deltamethrin and Achook respectively.However,in the ovary of treated fishes the total free amino acid content was significantly (P<0.05) reduced. In addition, DNA content was reduced to 26, 40 and 38% of controls (100%) in liver, ovary and muscle respectively after 16 days exposure to LC20 of Deltamethrin. The reduction in DNA content from the control was 37, 46 and 44% in liver, ovary and muscle respectively due to Achook at the same concentration and exposure period as that of Deltamethrin. Similarly, RNA contents were also reduced in the liver, ovary and muscle significantly after Deltamethrin and Achook exposure. It was observed that all the changes were concentration as well as time dependent. This study will reflect the potential role of these biochemical parameters for assessment of aquatic pollution as far as the natural pesticides are concerned.

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